Deep eutectic solvents pretreatment enhances methane production from anaerobic digestion of waste activated sludge: Effectiveness evaluation and mechanism elucidation.

Anaerobic digestion (AD) is a prevalent waste activated sludge (WAS) treatment, and optimizing methane production is a core focus of AD. Two DESs were developed in this study and significantly increased methane production, including choline chloride-urea (ChCl-Urea) 390% and chloride-ethylene glycol (ChCl-EG) 540%. Results showed that ChCl-Urea mainly disrupted extracellular polymeric substances (EPS) structures, aiding in initial sludge solubilization during pretreatment. ChCl-EG, instead, induced sludge self-driven organic solubilization and enhanced hydrolysis and acidification processes during AD process. Based on the extent to which the two DESs promoted AD for methane production, the AD process can be divided into stage Ⅰ and stage Ⅱ. In stage Ⅰ, ChCl-EG promoted methanogenesis more significantly, microbiological analysis showed both DESs enriched aceticlastic methanogens-Methanosarcina. Notably, ChCl-Urea particularly influenced polysaccharide-related metabolism, whereas ChCl-EG targeted protein-related metabolism. In stage Ⅱ, ChCl-Urea was more dominant than ChCl-EG, ChCl-Urea bolstered metabolism and ChCl-EG promoted genetic information processing in this stage. In essence, this study investigated the microbial mechanism of DES-enhanced sludge methanogenesis and provided a reference for future research.

Enhancing bioavailable carbon sources and minimizing ammonia emissions in distillery sludge and distiller's grains waste co-composting through deep eutectic solvent addition.

This study introduced two deep eutectic solvents, ChCl/oxalic acid (CO) and ChCl/ethylene glycol (CE), into a 34-day co-composting process of distillery sludge and distiller's grains waste to address challenges related to NH3 emissions. The addition of DES increased dissolved organic carbon by 68% to 92%, offering more utilizable carbon for microorganisms. SYTO9/PI staining and enzyme activity tests showed the CE group had higher bacterial activity and metabolic levels during the thermophilic phase than the control. Bacterial community analysis revealed that early dominance of Lactobacillus and Lysinibacillus in CE accelerated the onset of the thermophilic phase, reduced pile pH, and significantly decreased urease production by reducing Ureibacillus. Consequently, CE treatment substantially dropped NH3 emissions by 73% and nitrogen loss by 54%. Besides, CE fostered a more abundant functional microbial community during the cooling and maturation phases, enhancing deep degradation and humification of organic matter.

Removal of low concentration of perchlorate from natural water by quaternized chitosan sphere (CGQS): Efficiency and mechanism research.

In this study, an innovative approach utilizing betaine as a raw material was employed to effectively modify the surface of chitosan with quaternary ammonium groups. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectrometer (FTIR) characterization showed that the quaternary ammonium groups on betaine were successfully loaded on the chitosan surface. The effects of dosage, pH, initial perchlorate concentration, temperature and co-existing anions on the removal efficiency of perchlorate were investigated. The saturated adsorption capacity of CGQS was 35.41 mg/g under natural condition. The impact of initial perchlorate concentrations and column flow rates on the column adsorption experiments were investigated, as well as natural water tests. Sterilizing performance experiments of CGQS were carried out innovatively. Under the condition of initial concentration of 0.5 mg/L, 9 BV/h (bed volume per hour), the effluent natural water was up to standard (≤0.07 mg/L) with a treatment capacity of 210 BV/g, and the sterilizing rate of CGQS was up to 97.02%. The proposed adsorption mechanisms involved surface pore adsorption, electrostatic adsorption of quaternary ammonium groups, and ion exchange between chloride and perchlorate ions. The CGQS prepared in this work had great potential for treating trace perchlorate contamination in natural water.

The impact of dissolved organic matter on the photodegradation of tetracycline in the presence of microplastics.

Microplastics (MPs), an emerging class of pollutants, significantly impact the photoconversion dynamics of tetracycline (TC). But the effect of prevalent dissolved organic matter (DOM) on TC photodegradation in the presence of MPs remains a gap in current research. In this study, the photoconversion behavior and mechanism of TC under simulated sunlight conditions were systematically investigated, both in the presence of DOM and in combination with polystyrene (PS) MPs. The results demonstrated that both DOM and MPs enhanced the photodegradation of TC when compared to its direct degradation. However, DOM, particularly humic acid (HA, 10 mg/L), exhibited a more pronounced enhancing effect on TC photodegradation within 1 h reaction, regardless of the presence or absence of MPs, reaching up to 80%. In reaction systems involving TC-HA and TC-HA-PS, the primary contributors to TC degradation were direct photolysis and HA photosensitization (free radical reactions). Conversely, photosensitization effects were not significant in the presence of fulvic acid (FA). Furthermore, even under dark reaction conditions, HA exhibited a 10% degradation effect on TC. Quenching experiments and electron spin resonance (ESR) results indicate that dark reaction processes involve free radical reactions. Additionally, toxicity test results showed a reduction in the acute toxicity of TC photodegradation products, yet the long-term cumulative risks to organisms deserved attention. In general, this investigation significantly advances our understanding of the intricate photoconversion behavior of TC in the presence of coexisting chemical components.

Effect of polystyrene microplastics on tetracycline photoconversion under simulated sunlight: Vital role of aged polystyrene.

Photoconversion of tetracycline (TC) has been widely reported. However, the effect of microplastics (MPs) on TC conversion kinetics and mechanism has rarely been discussed. In this study, we investigated the effect of (aged) MPs on TC degradation under simulated sunlight and elucidated the underlying mechanism. Our findings demonstrated that the physical and chemical properties of polystyrene (PS), such as particle size, surface groups, and morphology, were significantly altered after aging. Moreover, photoconversion efficiency of TC was suppressed with the spiking of aged PS, while virgin PS showed an opposite tendency. The photodegradation reaction for photosensitization of PS involved 1O2 and HO·. The light-screening effect of aged PS occupied predominance, weakening the direct UV-light absorption of TC and resulting in lower TC degradation efficiency. Additionally, triplet-excited state PS was generated after photon acceptance by aged PS, which could transfer energy to O2, leading to the production of 1O2. The toxicity test manifested that the direct impact of TC products on fathead minnow was ignorable, but long-term negative effects on growth deserved observation. This study enhances our understanding of the environmental fate of PS and TC under sunlight, and provides crucial reference information for better evaluating the potential risk of MPs and chemicals.

Treatment of sewage sludge hydrothermal carbonization aqueous phase by Fe(II)/CaO2 system: Oxidation behaviors and mechanism of organic compounds.

The Fe(II)/CaO2 system with a stable oxidant and a low-cost homogeneous activating agent has been considered as a prospective process for the disposal of wastewater. The system was constructed to treat sewage sludge hydrothermal carbonization aqueous phase (HTC-AP) in this study. As the hydrothermal temperature increased, the organics in the HTC-AP were first decomposed and then cyclized, while the Maillard reaction occurs throughout the stage. The oxidation efficiency of the Fe(II)/CaO2 system was related to the composition of organics in HTC-AP, and the removal of dissolved organic carbon (DOC) by the system was 38.56 % in the HTC-AP obtained by hydrothermal treatment at 220 °C. Redundancy analysis showed that the low molecular weight organics, hydrophobic acids, and hydrophobic neutral components were beneficial to DOC removal, while Maillard products and cyclization products were hard to be oxidized to CO2 and H2O. The CN functional group of the protein facilitated DOC removal, and some organics in HTC-AP were oxidized to acids and phenols. The energy input to remove DOC in Fe(II)/CaO2 system was 27.74 MJ per kg carbon. This study provides a low-energy consumption Fe(II)/CaO2 system for the post-treatment of HTC-APs and explores the applicability of the system.

Mechanistic insights into enhanced waste activated sludge dewaterability with Cu(II) and Cu(II)/H2O2 treatment: Radical and non-radical pathway.

Without extra adjustment of pH, the effects of cupric ions (Cu(II)) and hydrogen peroxide (H2O2) alone or in combination on sludge dewatering were studied. It showed good dewatering capability after treated by Cu(II) and Cu(II)/H2O2, which indicated by the capillary suction times (CST) decreased from 120.8 ± 4.7 s (control) to about 40 s, and the water content (Wc) of sludge cake dropped by about 10%. The results showed that the extracellular polymeric substances (EPS) were destroyed, which characterized by a significant decrease in the biopolymers' concentrations in tightly-bound EPS. Meanwhile, more rough and porous microstructures and higher zeta potentials were obtained after conditioned. Based on the changes of physicochemical properties of sludge, the variations of EPS, and the identification of reactive species, two distinct mechanisms of improved sludge dewatering were postulated. As for Cu(II) treatment, it was mainly due to the surface charge neutralization, strong cytotoxicity of Cu(I) produced by intracellular reduction of Cu(II), and pH decline caused by Cu(II) hydrolysis that improved sludge dewatering performance, which could be noted as a "non-radical pathway". When in combination with H2O2, hydroxyl radicals (·OH) produced by Cu(II)-catalyzed Fenton-like process played a dominant role in degrading sludge flocs and EPS, which could be regarded as a "radical pathway".

Effect of temperature on the sulfur fate during hydrothermal carbonization of sewage sludge.

To understand the effect of reaction temperature on sulfur during hydrothermal carbonization (HTC) of sewage sludge (SS), seven group of temperature (180-300 °C) were chosen to investigate the distributions and evolution of sulfur-containing compounds in hydrochar and the liquid products. Elemental analysis, X-ray photoelectron spectroscopy (XPS), and X-Ray powder diffraction (XRD) were used to characterize the distribution of sulfur in hydrochar. The concentrations of sulfate ions and sulfide were determined in the liquid sample. The experimental results showed that as the temperature increased, the O/C ratio decreased because of the improved carbonization degree of SS. After hydrothermal carbonization, 90% of the sulfur in SS remained in hydrochar. As the temperature increased, the amount of sulfur in the liquid, mainly in the form of sulfate ions, tended to decrease. However, the experimental results for the gas phase were the opposite of the liquid phase.

Fabrication of bean dreg-derived carbon with high adsorption for methylene blue: Effect of hydrothermal pretreatment and pyrolysis process.

This study explored the cooperative effects of hydrothermal or activators pretreatment and pyrolysis process on the chemical composition of bean dreg (BD)-derived carbon materials and its adsorption characteristics for methylene blue (MB) further. The results showed that hydrothermal assisted pyrolysis carbon with or without activator (HBK and HB) possess higher BET surface area than no hydrothermal assisted carbon (BKs and B, respectively). But surface area alone was not a critical factor contributing to MB adsorption, it also depends on strong electrostatic interaction and the hydrogen bonding interaction between N+ in MB combines with OH and COO- group of adsorbent surface by pH-dependent adsorption and pseudo-second-order model. Additionally, BK800 (pyrolysis at 800 °C) was identified as the most efficient adsorbent due to maximum adsorption capacity (434.78 mg g-1), and BDP (BD-derived polymer composite hydrogel), possessing excellent adsorption property and high reusability, made BK800 easier to separate from the regenerated dye solution.

Co-hydrothermal carbonization of food waste-woody biomass blend towards biofuel pellets production.

Co-hydrothermal carbonization of food waste-woody biomass blend was conducted to enhance the pelletization and hydrochar-fuel properties. The hydrochar was characterized by proximate, elemental analysis and HHVs, whilst energy consumption of pelletization, tensile strength, and combustion characteristics of hydrochar pellets were evaluated. Results showed that food waste (FW) blended with 0-50% mainly decreased H/C of hydrochar, while blend ratio from 75% to 100% mainly decreased O/C. When FW blended from 0% to 75%, the energy consumption for hydrochar palletization decreased about 12-17 J, whereas tensile strength of pellets increased about 2.4-5.5 MPa by formation of solid bridge when woody biomass (WS) ratio was increased. The hydrochar pellets from high ratio FW had decreased ignition temperature and maximum weight loss rate with wider temperature range, indicating the increased flammability and moderate combustion. These findings demonstrate that HTC of food waste-woody biomass blend was suitable for pelletization towards solid biofuel production.